High Content Screening

High Content Screening (HCS)

HCS uses a high-throughput live cell imaging format to screen compounds in complex cellular systems to identify new drug targets or new lead compounds. More predictive than classic plate-reader high-throughput screening (HTS), the high content imaging screening format provides data specifically at the target site, as well as any off-target effects, all in the same assay. Using cell painting multiplex techniques, you can even monitor multiple nodes in a cellular pathway.

With extensive experience in high-throughput screening, in-house image analysis algorithm design, and disease biology, our team can help you select the right cell type and assay parameters before initiating HCS. We develop assays for small molecule screening using our 850,000-compound collection and for RNAi screening using our proprietary adenoviral platform, SilenceSelect®. Current screening assays include apoptosis, protein phosphorylation, receptor degradation, marker expression, and many more.

High Content Analysis (HCA)

As you move further into development, you can use HCA to profile lead compounds in disease-relevant cell-based assays. Detailing cellular physiology in a complex cellular system, live cell imaging delivers predictive, translatable data. HCA monitors phenotypic changes such as morphology, cellular localization and proliferation that can be both visualized and quantified and in real time. HCA has progressed to include multicellular structures such as 3D spheroids and co-cultures as well as multiplexing to monitor multiple features within a microenvironment all in one well.

High Content Imaging (HCI) Assay Development

High content imaging is a powerful tool for drug discovery, but its application requires specialized equipment and expertise that few organizations have in-house.

High Content Screening – Agilent BioTek Imaging & Microscopy

• Augmented Microscopy

• Agilent BioTek instrumentation and software create unique augmented microscopy experience with the integration and automation of all steps from image capture to the creation of publication-ready data. There is no need for other software as Agilent BioTek Gen5 does it all quickly and simply:

• Images are automatically captured: end point, time-lapse, montage, z-stack

• Automated processing, including background flattening and deconvolution, optimizes images for downstream analysis

• Nuclear and cytoplasmic components are identified with primary and secondary masks

• Image analysis offers qualitative and quantitative data

• Annotation of single or sequential images is available on-screen, in real time

• Video clips can be generated from time-lapse sequences with a single click

• Augmented Microscopy creates a 'map' with key analytical information, helping you to make informed decisions faster.